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KMID : 0620919990310020071
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Experimental & Molecular Medicine
1999 Volume.31 No. 2 p.71 ~ p.75
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Induction of fibronectin gene expression by inhibitors of protein phosphatase yype 2B in normal and transformed fibroblasts
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In San Kim/Jung Hwa Rhew
Young Ah Shin/Byung Heon Lee/Rang Woon Park/In San Kim
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Abstract
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Two intracellular signal pathways mediated by cAMP and protein kinase C (PKC) were involved in the regulation of FN gene expression (Lee et al., Exp. Mol. Med. 30: 240, 1998). In this study, a possible involvement of protein phosphatase-dependent pathways in the regulation of FN gene expression was investigated by using protein phosphatase yype 2B (PP2B) inhibitors, cyclosporin A and ascomycin. Both cyclosporin A and ascomycin increased the levels of FN mRNA in WI-38 human lung fibroblasts and the SV40-transformed WI-38 cells but not in MC3T3-E1 osteoblasts. The expression of FN appears to increase from six hours up to 48 hours after treatment suggesting that it is not an immediate effect. In addition, this effect required a new protein synthesis. Neither cyclosporin A nor ascomycin affects the phorbol myristate acetate (PMA)-induced stimulation of FN gene expression and the same result occurred in vice versa suggesting the mechanism of PMA and cyclosporin A/ascomycin in the regulation of FN gene expression may share a common downstream pathway. Taken together, this study suggests that PP2B is involved in the regulation of FN gene expression in normal and transformed fibroblasts but not in osteoblasts.
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KEYWORD
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ascomycin, cyclosporin A, fibroblasts, fibronectin, protein phosphatase 2B,
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